CD25 antibody

Catalog No. ABIN181952

The Mouse Monoclonal anti-CD25 antibody has been validated for FACS. It is suitable to detect CD25 in samples from Sheep.

Quick Overview for CD25 antibody (ABIN181952)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 01-09-14

Product Details anti-CD25 Antibody

Specificity: This antibody is specific for CD25, also known as Interleukin-2 receptor alpha chain.

Purification: Affinity chromatography on Protein G

Immunogen: Con A-stimulated ovine lymph node cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 1 x 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 / IL2RA

Background: Ovine CD25 is expressed by activated T cells.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gene ID: 443435

NCBI Accession: NP_001009415

UniProt: P26898

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation